By Yutaka Kawarabayasi Ph.D. (auth.), Pratyoosh Shukla, Brett I. Pletschke (eds.)

ISBN-10: 813221093X

ISBN-13: 9788132210931

ISBN-10: 8132210948

ISBN-13: 9788132210948

Enzyme expertise is one the main promising disciplines in glossy biotechnology. during this publication, the purposes of a wide selection of enzymes are highlighted. present experiences in enzyme know-how are concentrated in the direction of the invention of novel enzymes (termed “bio-discovery” or “bio-prospecting”) and the identity and elucidation of novel pathways of those novel enzymes with emphasis on their business relevance. With the improvement of molecular concepts and different bioinformatics instruments, the time to combine this topic with different fields within the existence sciences has arrived. A quick enlargement of the information base within the box of enzyme biotechnology has happened during the last few years. a lot of this enlargement has been pushed via the bio-discovery of many new enzymes from a variety of environments, a few severe in nature, via next protein (enzyme) engineering. those enzymes have discovered quite a lot of purposes, starting from bioremediation, bio-monitoring, biosensor improvement, bioconversion to biofuels and different biotechnologically vital value-added items. Hydrolases represent a huge part of the worldwide annual profit generated by way of business enzymes and the emphasis has accordingly been put on those enzymes and their purposes. With the sizeable curiosity of researchers lively during this region, this e-book will serve to supply info on present points during this box of analysis. within the present version, the contributions of many varied themes in the direction of constructing new instructions of analysis within the quarter of enzyme biotechnology are defined. This ebook serves to supply a different resource of knowledge to undergraduates, put up graduates and doctoral classes in microbiology and biotechnology in addition to allied existence sciences. the current variation of the booklet covers all very important components of enzyme biotechnology i.e. the big variety of enzymes within the box of enzyme biotechnology and their business purposes, new equipment and state of the art details on glossy tools of enzyme discovery. This booklet will act nearly as good source on lots of the present features of enzyme expertise for all scholars engaged in bioengineering and biotechnology.

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Int J Food Eng 6, article 4 Martinez-Villaluenga C, Cardelle-Cobas A, Corzo N, Lano A, Villamiel M (2008) Optimization of conditions for galactooligosaccharide synthesis during lactose hydrolysis by beta-galactosidase from Kluyveromyces lactis (Lactozym 3000 L HP G). Food Chem 107:258–264 Matella NJ, Dolan KD, Lee YS (2006) Comparison of galactooligosaccharide production in free-enzyme ultrafiltration and in immobilized-enzyme systems. J Food Sci 71:C363–C368 Maugard T, Gaunt D, Legoy MD, Besson T (2003) Microwave-assisted synthesis of galactooligosaccharides from lactose with immobilized betagalactosidase from Kluyveromyces lactis.

Hemicellulose is associated with cellulose and lignin and forms about 30 % of H. Soni • N. Kango (*) Department of Applied Microbiology and Biotechnology, Dr. com the lignocellulosic biomass. Lignocellulose is abundant and represents one of the major natural renewable resources and a dominating waste material from agriculture. This renewable resource can be used in several industries, including the pharmaceutical, biofuel, and pulp and paper industries, and many more (Kango et al. 2003; Kango 2007).

2011; Mayer et al. 2004), and galactosyl-polyhydroxyalcohols (Irazoqui et al. 2009; Klewicki 2007), using free and immobilized enzyme. The β-galactosidase from A. oryzae has been immobilized by different strategies including entrapment in alginate (Freitas et al. 2011), covalent attachment onto various carriers (Gaur et al. 2006; Huerta et al. 5 U/ml, 40 °C Neri et al. 2011; Sheu et al. 1998) or combined ionic adsorption and cross-linking (Guidini et al. 2011). Specificity of A. oryzae β-Galactosidase We have studied the synthesis of GOS catalyzed by a preparation of β-galactosidase from A.

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Advances in Enzyme Biotechnology by Yutaka Kawarabayasi Ph.D. (auth.), Pratyoosh Shukla, Brett I. Pletschke (eds.)

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